PCR Purification Kit

May 23, 2016

BlueskyReddit
Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Qiagen Pvt Ltd

Product Name:

QIAquick PCR Purification Kit

Catalog Number:

28104

I am working on the development of molecular methods for the detection of pathogens in the environment. I prefer this kit for the purification of PCR products to be used for further downstream processing like cloning and sequencing of the amplified DNA.

Experimental Design and Results Summary

Application

Purification of PCR product which is required for further cloning and sequencing

Starting Material

None

Protocol Overview

The protocol mentioned in manual was easy and quick

Tips

If eluting DNA in sterile water, store it at -20 degree celsius

Results Summary

Results obtained were of good quality

Additional Notes

None

Image Gallery

Summary

The Good

The kit eliminate the need of excising bands from agarose gels to purify the DNA fragment

The Bad

Overall yield of purified DNA is low

The Bottom Line

The kit is very useful for the purification of PCR products to be used further for cloning and sequencing.

Join the discussion