eBioscience
Fixable viability dye efluor 450
65-0863-18
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This antibody was selected because of the ease of use--following eBioscience's protocol exactly. This stain was used for every experiment I needed to fix or not fix cells.
Flow Cytometry
Mouse Lymphocytes
Followed manufacturer's protocol. Used at 1:1000 in PBS for 30minutes.
NA
Fortessa
The live dead stain is very consistent. The two populations are distinct.
None
Distinct live/dead populations
Long incubation time, have to store at -80C
Very good live/dead stain for fixing and non fixing protocols.