The Fastest Library Preparation

PCG Molecular
Molecular Diagnostics
Director of Molecular Diagnostics

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

QIAseq 1-step Amplicon Library Kit

Catalog Number:

180412

This kit allows us to shorten NGS library preparation time.

Experimental Design and Results Summary

Application

For Illumina based sequencing MiSeq, NextSeq, HiSeq.

Starting Material

PCR amplicon by using Qiagen generead amplification kit

Protocol Overview

Only one step preparation and reaction includes all 3 steps procedures such as end-repair, A-addition, Adaptor ligation and 3 times purification steps.

Tips

Just add 10ng - 100ng Amplification DNA

Results Summary

After the amplification, yield and quality is the same as 3-step preparation. It helps us save lots of labor and time.

Additional Notes

Image shows a picture of tapestation results of pre-capture amplification after 1-step ligation reaction. Results show that 1-step ligation reaction is as good as 3-step ligation, which means we can save lots of time and labor.

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Summary

The Good

It is easy and convenient.

The Bad

Not much

The Bottom Line

Low cost, saves lots of labor and time. Good quality.

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