Great Leukocyte Marker

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biotechnologies

Product Name:

CD45 APC/Cy7

Catalog Number:

25-0459

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody stains leukocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

Bright and good Separation

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great separation between positives and negatives

The Bad

Has a fair amount of decoupling

The Bottom Line

Great antibody unless you are using something in APC, as CD45 APC/Cy7+ are VERY bright in APC

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