Great Cytotoxic T-Cell Marker from BD Biosciences

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD8a BUV496

Catalog Number:

564804

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody stains CD8+ Cytotoxic T-Cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

Used in dump channel with viability dye, negatively selects non-viable and cytotoxic T-cells out

Additional Notes

None

Image Gallery

Summary

The Good

Very Bright, Great Separation

The Bad

No problems

The Bottom Line

Fantastic Ab

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