VE-Cadherin in Lung Microvessels

March 22, 2016

Physiology
The University of Tennessee Health Science Center
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Company:

Santa Cruz

Product Name:

VE-Cadherin

Catalog Number:

SC-9989

We determined expression of endothelial VE-cadherin in microvessels of intact blood-perfused lungs by in situ immunofluorescence. (Ref Am J Physiol Lung Cell Mol Physiol (July 10, 2015). doi:10.1152/ajplung.00211.2014)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Lung

Primary Incubation

30 min

Blocking Agent

5% FBS

Secondary Incubation

30 min

Tertiary Incubation

NA

Detection

Confocal microscopy

Results Summary

Ref journal article: Changes in endothelial Cx43 expression inversely correlates with microvessel2 permeability and VE-cadherin expression in endotoxin challenged lungsKathirvel Kandasamy1, Rachel Escue1, Jayeeta Manna1, Adebowale Adebiyi1 and Kaushik 5 Parthasarathi1,2Am J Physiol Lung Cell Mol Physiol (July 10, 2015). doi:10.1152/ajplung.00211.2014

Additional Notes

None

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Summary

The Good

Great immunofluorescence image

The Bad

None

The Bottom Line

Ease of use

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