Thermo Fisher Scientific
Rhodopsin Antibody (1D4)
MA1-722
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Rhodopsin, is a G-protein family which is present in the photoreceptor layer where rods and cone cells are present.This protein detect the degree of lightness entering the retina and their sensitivity is dependent on the amount of Rhodopsin present which is itself generated within the cells.
Immunofluorescence
Paraffin rat retinal sections.
1/1000 dilution in 3% BSA, overnight at four degrees on shaker.
5% BSA/1 hr at RT
3%PBS/1 hr at RT
None
Chemiluminescence
Observed rhodopsin expression in photoreceptor layer of various rat retinal sections. Dilution: 1:1000 (IF).
Take the 4µ paraffin retina sections then transfer into xylene and gradient alcohols (90, 70, and 60%). Next add proteinase-K/10min. Wash with PBS. Block the sections with 5% PBS/1 hr. After washing, go for incubation with this Ab in 3%PBS overnight/4oC. Next day wash and incubate with 2OAb in 3%PBS/1 hr. Then wash and mount with DAPI. Check in microscope.
Very specific to rhodopsin protein
Need more volume of antibody for IF
Very low cross reactivity of this Ab makes good results