Synaptophysin Stains Retina Tissue

March 21, 2016

Cancer biology
The Jackson Laboratory
Post doc

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Company:

Sigma-Aldrich

Product Name:

Anti-Synaptophysin

Catalog Number:

S5768

We used Anti- Synaptophysin to stain synapses in mouse retina frozen and formalin fixed tissues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse Retina

Primary Incubation

1: 300 in blocking serum containing BSA (0.5%) in PBS

Blocking Agent

Blocking serum containing BSA (0.5%) in PBS, 1hr RT

Secondary Incubation

1:1000 Goat Anti mouse Alexa 568 in blocking serum

Tertiary Incubation

NA

Detection

Epifluorescence microscopy

Results Summary

The image shows specific staining of synaptic vesicles staining by synaptophysin in the synapses of photoreceptor and bipolar neurons.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong signal with high dilution

The Bad

None

The Bottom Line

This antibody will be best to stain the synaptophysin/ synaptic layer of the retina

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