One Compensation Bead for All Isotypes

University of Rochester
MBI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

OneComp eBeads

Catalog Number:

01-1111-42

Compensation beads were used to determine spillover between fluorphores.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

OneComp Compensation Beads

Protocol Overview

Incubated 1:200 of various antibody isotypes. Detection was done on LSRII Flow Cytometer.

Tips

None

Results Summary

Beads stained with all tested isotypes and all tested fluors.

Additional Notes

None

Related Categories

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Summary

The Good

Stains brightly with multiple isotypes.

The Bad

High background off violet laser.

The Bottom Line

Works with multiple antibody isotypes allowing the lab to only stock one compensation bead vial.

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