Human CD4 (PE/Dazzle™ 594)

Immunology
Universitätsklinikum Hamburg-Eppendorf
BTA

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-human CD4

Catalog Number:

300548

We are staining this antibody on whole cord blood in a 15 colour panel. It works very well to detect Th cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole cord blood

Primary Incubation

0.1µL CD3 in 50µL FACS buffer added to 50µL blood for 30min at RT

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR Fortessa

Results Summary

This antibody works very well to detect human Th-cells and gives a clear separation between positive and negative populations.

Additional Notes

None

Related Categories

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Summary

The Good

The antibody gives very good results in low concentrations.

The Bad

None

The Bottom Line

This antibody is good for multi-color panel.

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