Great STAT3 Antibody

Research
University of North Carolina at Chapel Hill
Laboratory Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Stat3 (79D7) Rabbit mAb

Catalog Number:

4904

Our lab works with all of the STAT proteins in the context of kidney cancer, and we are often blotting for STAT3. We chose to give this Cell Signaling antibody a try for western blotting 786-0 cell lysates and haven't looked back.

Experimental Design and Results Summary

Applications

Western Blot

Sample

786-0 Cell Lysates

Primary Incubation

1:1000 Dilution in 5% BSA in 1X TBS-T at 4C Overnight

Blocking Agent

5% Milk in 1X TBS-T

Secondary Incubation

1:5000 dilution in 5% Milk in TBS-T for 1 hour at RT

Tertiary Incubation

NA

Detection

Autoradiography Film using ECL alone

Results Summary

This antibody worked out great. We had no problems detecting STAT3 protein levels in 786-0 cells and several other kidney cancer cell lines. It comes out surprisingly clean on film, and doesn't require anything other than ECL.

Additional Notes

None

Related Categories

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Summary

The Good

Great quality results

The Bad

Expensive

The Bottom Line

This antibody is worth giving a try if you work with STAT3. Give Cell Signaling a chance, purchase this antibody, and be pleased with your results. I highly recommend it.

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