Bethyl Laboratories
RbBP7 Antibody
A300-958A
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I am investigating functions of chromatin modification complexes in RNA expression. The goal of this experiment was to test the specificity of the antibody to detect RbBP7 expression in 293 cells.
Western Blot
293 Whole Cell Extract
1:1000 dilution overnight in 5% milk buffer at 4C
5% milk in TBS
1:10000 anti-rabbit secondary at RT for 1 hour
NA
ECL
The results show a strong band at 50kDa and a weak minor band around 70kDa
None
Strong band can be obtained at the correct molecular weight
Non-specific band around 70kDa
This antibody works for RbBP7 detection