Violet Laser Cell Tracking Dye

University of Rochester
MBI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CellTracker™ Violet

Catalog Number:

C10094

Thymocytes were stained for injection into the peritoneal cavity of mice. To be compatible with our previously designed flow panel a violet laser dye was selected.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Mouse Thymocytes

Protocol Overview

Cells were incubated with 5uM CellTracker Violet in PBS @ 37*C for 20 minutes. Reaction was quenched with media containing FBS.

Tips

None

Results Summary

Very bright staining of thymocytes in the Violet laser 525/50 filter.

Additional Notes

None

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Summary

The Good

Staining is time and concentration dependent so it's very easy to optimize.

The Bad

More expensive compared to other CellTracker dyes

The Bottom Line

A great dye for in vivo cell tracking if you have an opening on the violet laser.

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