FITC Anti-Mouse CD45

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD45

Catalog Number:

103107

LPS was instilled in mouse lungs by intranasal instillation. Bronchoalveolar lavage was performed 24 h later, and the cells were isolated and stained for flow cytometry. CD45 antibody was used to select hematopoietic cells to further characterize the recruited cell population. Used this product because of their robust staining and clear results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bronchoalveolar lavage cells

Primary Incubation

1:100 in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

Clearly identified CD 45 positive cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good separation

The Bad

None

The Bottom Line

Highly recommend using this product for flow cytometry of primary cells

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