eBioscience
Anti-Mouse Ly-6C eFluor® 450
48-5932-80
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Mice were intraperitoneal injected with heat-killed E. coli and peritoneal lavage recovered. Immune populations were identified by flow cytometry of total peritoneal cells.
Flow Cytometry
Peritoneal cells - 8h after heat-killed E. coli injection
1:200 Ly6c-eFlour450 1:400 Ly6g-APC/Cy7 - 30 minutes on ice
1:100 FcBlock - 5 minutes on ice
NA
LSRII Flow Cytometer
Plotting Ly6c-eFlour450 by Ly6g-APC/Cy7 allows clear identification of monocytes (Ly6ChiLy6G-) and neutrophils (Ly6CintLy6G+) from peritoneal cells.
Gated on live, single cells
Bright staining
None
This antibody is a good choice for identification of murine monocytes when combined with Ly6g.