Bright, Inexpensive CD11b Antibody

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

FITC Anti-Human/Mouse CD11b

Catalog Number:

35-0112

Browse Similar Products: CD11b Flow Cytometry Antibodies

CD11b and F480 were used to identify macrophages from naive and thioglycollate injected animals.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Naive and D3 Thioglycollate Elicited Peritoneal Cells

Primary Incubation

1:400 CD11b-FITC 1:200 F480-BV421 - 30 minutes on ice

Blocking Agent

1:100 FcBlock - 5 minutes on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSRII Flow Cytometer

Results Summary

Macrophages from naive and thioglycollate treated animals were easily detected by CD11b/F480 double positive staining. CD11b is sufficiently bright that macrophages could also be identified by CD11bhi/SSChi.

Additional Notes

None

Related Categories

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Summary

The Good

Inexpensive, bright staining

The Bad

None

The Bottom Line

CD11b-FITC from Tonbo performs as well as other CD11b antibodies I have tested from other companies.

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