Ly6G Antibody Works great!

Department of Pediatrics
University of California, San Diego
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Leinco Technologies, Inc

Product Name:

Anti-mouse Ly6G DyLight 488

Catalog Number:

L287

This antibody was used to stain cells from murine bone marrow and murine lung (during infection) - looking at Ly6G to identify neutrophils.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow, blood and lung

Primary Incubation

1:100 or 1:200, 4C or ice, 20 min

Blocking Agent

FcBlock in PBS or FACS buffer

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry, FITC channel

Results Summary

Great! Works as expected, used for surface staining with our standard flow cytometry protocol. Clear positive and negative populations, staining looks good. Note: the gate drawn on the sample image shown is labeled "FITC" but the Ab was indeed DyLight488. Sample shown is murine bone marrow, stained for 20 minutes on ice, and then fixed in 4% PFA for 20 minutes, then washed in FACS buffer (we have to fix samples due to biosafety reasons, but this is not necessary).

Additional Notes

None

Image Gallery

Summary

The Good

Works just as well as any other flow antibody, bright / clear staining.

The Bad

None

The Bottom Line

Quality product, works as expected!

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