Good Specific Staining Antibody for gamma delta TCR

Rheumatology
University of California
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti gamma delta TCR APC

Catalog Number:

118115

Our experiment required depletion of BALB/c mice with the anti gamma delta TCR specific antibody in mice injected with a biological reagent. Therefore the next step required the identification of small traces of remaining cells with a specific antibody to make sure that the depletion worked well with the depleting antibody. Good Anti-gamma delta TCR specific antibody in multiparametric flow cytometric analysis was used to distinguish gamma delta TCR positive cells from alpha beta TCR positive T cells and myeloid cells. More than 5 colors were used and this antibody color was good enough to be detected in the channel with little or no leakage in any other channel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen cells

Primary Incubation

2 hrs at 1:500 dilution

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometrically on FL4

Results Summary

Specific, good signal was obtained for GD+T cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Cheap and good color

The Bad

Sometimes leaks in AF-700

The Bottom Line

Overall good if properly compensated in different channels

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