Akt Antibody from Cell Signaling #9272S

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt Antibody

Catalog Number:

9272S

This antibody was used to detect total Akt1 expression and used as a control for phosphorylated Akt S473 (Cell Signaling #4051S).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:2000, 2 hours, RT

Blocking Agent

Non-fat milk

Secondary Incubation

1:5000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody perfectly detected total Akt expression levels by western blot in human breast cancer cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Signal is strong and specific.

The Bad

NA

The Bottom Line

This is a perfect antibody for western blot.

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