Good Antibody to Detect aSMA

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Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-alpha smooth muscle Actin antibody

Catalog Number:

ab7817

Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the presence of ASMA+ cells in a PP section.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse PP section

Primary Incubation

1ul/100ul; 1 hour at RT

Blocking Agent

10% goat serum

Secondary Incubation

AlexaFluor 647 goat anti-mouse: 1:500; 30 min; RT

Tertiary Incubation

NA

Detection

Leica confocal microscope

Results Summary

You can see a nice staining of aSMA+ cells.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Highly recommended antibody

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