Good Antibody to Detect VCAM-1+ Cells

IBIMER
CIBM
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD106 (VCAM-1) eFluor® 660

Catalog Number:

50-1061-82

Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the presence of VCAM-1+ cells in a PP section.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse PP section

Primary Incubation

1ul/100ul; 1 hour at RT

Blocking Agent

10% goat serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Leica confocal microscope

Results Summary

You can see a nice staining of VCAM-1+ cells, showing a stromal pattern.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Highly recommended antibody

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