Good Antibody to Detect B Cells

IBIMER
CIBM
Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488

Catalog Number:

53-0452-80

Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the presence of B cells in a LN section.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse PP section

Primary Incubation

1ul/100ul; 1 hour at RT

Blocking Agent

10% goat serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Leica confocal microscope

Results Summary

You can see a nice staining of B cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Highly recommended antibody

Share your experience with other scientists. Write a Review! »

Join the discussion