Jackson ImmunoResearch Laboratories, Inc.
Goat IgG FITC
005-090-006
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The aim of this experiment was to determine the proportion of RBC'S bound to different immunoglobulin from dogs that have immune mediated hemolytic anemia. This antibody was used as an isotope control for the Goat anti dog IgG, IgM and C3 complement.
Flow Cytometry
Whole blood in EDTA
100ul of 1:100diluted 1mg/ml antibody with the 100ul of washed cells incubated for 30mins in the dark.
NA
FACS CAlibur
This isotype control works well in separating the negative population from the positive. It is able to show us which of the bound RBC's are true positive binding.
None
Works well as an isotope for for experiments.
Good Isotype.