Good General Purpose B Cell Marker

February 22, 2016

Research Participation Program
Oak Ridge Institute for Science and Education
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Biotin anti-mouse CD19

Catalog Number:

115504

We have an immunology lab that studies the phenotypes of B cells in mice. This antibody is very effective at labeling B cells from total splenocyte preparations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Live murine splenocytes, red blood cells lysed

Primary Incubation

1:100 dilution anti-mouse CD19 on ice for 10 minutes

Blocking Agent

1x PBS + 2% FBS + 2mM EDTA

Secondary Incubation

1:100 dilution BV421-streptavidin

Tertiary Incubation

NA

Detection

BD LSR Fortessa

Results Summary

CD19+ cells are clearly labelled.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable, strong labeling of splenic mouse B cells

The Bad

No drawbacks observed.

The Bottom Line

I would recommend this antibody for reliable labeling of CD19+ B cells.

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