Bethyl Labs
Goat anti dog IgM FITC
A40-116F
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The aim of this experiment was to determine the proportion of RBC'S bound to IgM immunoglobulin from dogs that have Immune mediated hemolytic anemia. This antibody serves the purpose of detecting such bound RBC's.
Flow Cytometry
Whole blood in EDTA
100ul of 1:100diluted 1mg/ml antibody with the 100ul of washed cells incubated for 30mins in dark.
NA
FACS CAlibur
The results generated by flow cytometry are in the form of percent positive or negative. In the case of dogs that are suspected of IMHA, we do see a positive population of cells bound to IgM. The percentage of these cells varies from dog to dog.
None
Good antibody
Works well.