Easy to Use Lentivirus P24 Assay

University of Miami
Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Clontech

Product Name:

Lenti-X GoStix

Catalog Number:

631243

The Lenti-X GoStix is a simple, easy to use product to ascertain viral titre via P24. If your IFU is greater than 0.5 x 10^5 you will see a band and if not you will not see a band. There is also a positive control that comes with every test. It reminds me of a pregnancy test.

Experimental Design and Results Summary

Application

Lentivirus Titre Assay

Starting Material

None

Protocol Overview

Add 20 ul of viral supernatent followed by 4 drops of "chase buffer" wait a few minutes and determine if you see a band or not.

Tips

Follow the protocol

Results Summary

I have used this on multiple virus preps and the test band shows up in a couple minutes along with the control band. I have used the same viruses to transduce cells downstream so I believe the band is a good measure of if you have virus or not.

Additional Notes

None

Image Gallery

Summary

The Good

Really simple to use

The Bad

Does not give an exact number, just a greater than or not

The Bottom Line

This is a very easy to use test to look at viral titre.

Join the discussion