R&D Systems
Human MFG‑E8 PE‑conjugated Antibody
IC27671P
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Browse Similar Products: MFG-E8 Flow Cytometry Antibodies
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the MFG-E8 expression by human fibroblasts and found this Ab really useful.
Flow Cytometry
Human fibroblast
1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT
1% BSA + 4% human serum
NA
FACScalibur
Gating on forward/side scatter alone gives a uniform population with respect to MFGE8. MFGE8 is shown in blue and isotype control is shown in red.
Cells were fixed in 4%PFA for 30 min at 4ºC and washed with 0,5% saponine in PBS
No background
None
It is an easy to use antibody that delivers good results