HLA-G Antibody (MEM-G1)

February 05, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-HLA G antibody [MEM-G/1]

Catalog Number:

ab7759

Our work involves the characterization of human fibroblasts from placenta. We analyzed the HLA-G expression by WB in JEG-3 and Jurkat cells and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Western Blot

Sample

JEG-3 and Jurkat lysate

Primary Incubation

1:1000, O/N at 4C

Blocking Agent

Milk

Secondary Incubation

1:5000 anti-mouse HRP antibody, 1 hr at RT

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

The antibody detected a single band of protein at around 40 KD.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results.

Share your experience with other scientists. Write a Review! »

Join the discussion