Excellent Anti-Mouse F4/80 Antibody

February 08, 2016

BlueskyReddit
MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Tonbo Biosciences

Product Name:

FITC Anti-Mouse F4/80 Antigen

Catalog Number:

35-4801

Browse Similar Products: F4/80 Flow Cytometry Antibodies

Peritoneal leukocytes were evaluated in naive mice. Macrophages were identified by CD11bhiF480hi expression.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Total Peritoneal Leukocytes

Primary Incubation

1:200 F480-FITC - 30 mins on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II (100mW Blue Laser)

Results Summary

Macrophages are clearly identifiable by CD11b/F480. CD11bint/F480int populations can also be clearly identified and used to identify eosinophils.

Additional Notes

Gated on CD45+7AAD-Singlets

Related Categories

Image Gallery

Summary

The Good

Price ($34 for 25ug) and staining results

The Bad

None

The Bottom Line

This F480 antibody performs well. We also tested Tonbo's F4/80-FITC offering against another major antibody manufacturer and found the results comparable.

Share your experience with other scientists. Write a Review! »

Join the discussion