Bright Siglec-F Antibody

February 05, 2016

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen

Product Name:

PE Rat Anti-Mouse Siglec-F

Catalog Number:

562068

The goal of these studies were to identify different immune populations in the peritoneal cavity following injection with heat-killed E. coli. This antibody was purchased because it was one of the few Siglec-F antibodies available at the time (more colors and clones have since become available).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Total Inflammed Peritoneal Leukocytes

Primary Incubation

1:400 SiglecF-PE 30 mins on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II (120mW Green Laser)

Results Summary

Eosinophil infiltration can be seen following heat-killed E. Coli injection into the peritoneal cavity of mice. Siglec-F-PE from BD Pharmingen, in combination with CD11b made identification of these cells simple with no ambiguity.

Additional Notes

Gated on CD45+7AAD-Singlets

Related Categories

Image Gallery

Summary

The Good

Bright staining, clear postive population

The Bad

None

The Bottom Line

This antibody/clone works well. Recently BD Pharmingen has begun conjugating this antibody to more exotic colors making it a great antibody to add to multi-color panels for identification of Siglec-F+ cells.

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