Real Time PCR

University of California
Internal Medicine
Post Doc

Overall

Performance

Ease-of-Optimization

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Company:

SA Biosciences

Product Name:

Stratagene Mx3000P QPCR System

Catalog Number:

QPCR system

Our study was aimed at determining the levels of message of different cytokines in variously treated tissues of mice. This machine is part of core facility and hence the primary reason for use.

Experimental Design and Results Summary

Application

Real time PCR

Starting Material

cDNA from various tissues

Protocol Overview

Same as in real time PCR applications and run protocol as mentioned in the template of the machine

Tips

Keep looking for samples to run while machine is running as machine fails to display results sometimes.

Results Summary

While real time results obtained seemed accurate, machine does have an inherent problem with saving the data sometimes. I felt the QPCR results were saved but after 2 hours of running, when I came back, the results were nowhere to be found. Run seemed fine but data cannot be saved by machine sometimes or saves in folders that are linked elsewhere and are hard to find.

Features Summary

Run should be monitored

Additional Notes

None

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Summary

The Good

Simple and easy to use

The Bad

Requires monitoring

The Bottom Line

Overall ok if you are going to keep an eye on your run.

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