Bright CD204 Option

February 02, 2016

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD204-PE, mouse (clone: REA148)

Catalog Number:

130-103-018

Mice were injected with 250ug zymosan IP and peritoneal cells were harvest 18 hours later. The goal was to assess CD204 levels on peritoneal macrophages post-inflammation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Total Inflammed Peritoneal Leukocytes

Primary Incubation

1:10 anti-CD204-PE - 30 mins on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II (120mW Green Laser)

Results Summary

Great separation between macrophages and non-macrophages with a staining index of 243. Easily used to identify CD204+ macrophages during inflammation.

Additional Notes

None

Related Categories

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Summary

The Good

Inexpensive and no need to titrate. hIgG1 isotype does not need FcBlock

The Bad

You have to use this antibody at 1:10 which is inconvenient if staining with multiple antibodies

The Bottom Line

Good price ($50 for 100 test) and essentially no background staining on CD204neg cells compared to unstained.

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