Bright, Inexpensive CD4 Antibody

February 01, 2016

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

CD4 PE

Catalog Number:

50-0041

Browse Similar Products: CD4 Flow Cytometry Antibodies

Thymocytes were isolated from a 6-7 week old mouse and stained with CD4 and CD8a to assess thymic T cell development.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Thymocytes

Primary Incubation

1:400 CD4-PE - 30 mins on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR II

Results Summary

Bright, specific staining of CD4. Combined with CD8a it was easy to identify DP, SP and DN thymocytes.

Additional Notes

This antibody is only $17 for 25ug at the time of review.

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Summary

The Good

Price and quality results

The Bad

None

The Bottom Line

A great antibody at a great price, well worth keeping a tube stocked in the lab

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