Bright, Inexpensive CD8 Antibody

MBI
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

CD8a FITC

Catalog Number:

35-0081

Browse Similar Products: CD8a Flow Cytometry Antibodies

Total thymocytes were isolated and stained with anti-CD4 and anti-CD8a to grossly assess thymic development.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Thymocytes

Primary Incubation

1:400 CD8a-FITC - 30 mins on ice

Blocking Agent

1:100 FcBlock - 10 mins on ice

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSRII flow cytometer

Results Summary

Single positive, double positive and double negative cells were easily distinguished when combining CD8a-FITC with CD4-PE. At the time of writing this antibody is $17 for 25ug.

Additional Notes

None

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Summary

The Good

Price and quality results

The Bad

None

The Bottom Line

This antibody works well to identify CD8a+ positive cells, a must have antibody to keep around the lab.

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