CD276 Expression by Human Fibroblasts

February 17, 2016

BlueskyReddit
BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-human CD276 (B7-H3) Antibody

Catalog Number:

331605

Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CD276 expression by human fibroblasts and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human fibroblast

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Gating on forward/side scatter alone give a uniform population with respect to CD276. CD276 is showed in blue and isotype control is showed in red.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results

Share your experience with other scientists. Write a Review! »

Join the discussion