CD273 Expression by Human Fibroblasts

February 16, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse CD273 (B7-DC, PD-L2) Antibody

Catalog Number:

107205

Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CD273 expression by human fibroblasts and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human fibroblasts

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Gating on forward/side scatter alone give a uniform population with respect to CD273. CD273 is showed in blue and isotype control is showed in red

Additional Notes

None

Image Gallery

Summary

The Good

No background

The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results

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