CD54 Expression by Human Fibroblasts

February 03, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD54 Antibody

Catalog Number:

353108

Our work involves the characterization of human fibroblasts from different tissues. We analyzed CD54 expression by human fibroblasts and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human fibroblast

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Gating on forward/side scatter alone give a uniform population with respect to CD54. CD54 is shown in blue and isotype control is shown in red.

Additional Notes

None

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

It is an easy to use antibody that delivers good results

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