HLA-DR Expression by Human Fibroblasts Treated with INFg

January 22, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-human HLA-DR Antibody

Catalog Number:

307622

Our work involves the phenotypic and functional characterization of human fibroblasts from different tissues. We analyzed HLA-DR expression of human fibroblasts after INFg treatment and we found this Ab really useful. This is a nice Ab to detect HLA-DR by confocal microscopy.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human fibroblast

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Confocal microscopy

Results Summary

Human fibroblasts treated with INFg O/N express HLA-DR

Additional Notes

None

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Summary

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The Bottom Line

Human fibroblasts treated with INFg

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