EGFR Antibody from Sigma-Aldrich

February 03, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

EGFR Antibody

Catalog Number:

E2760

Browse Similar Products: EGFR Western Blot Antibodies

This antibody was used to detect expression of total EGFR in human HNSCC cell lines.

Experimental Design and Results Summary

Applications

Western blot

Sample

Human cancer cells whole lysates

Primary Incubation

1:2000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:5000, 1 hour, RT

Tertiary Incubation

NA

Detection

Enhanced chemiluminescence detection kit

Results Summary

This antibody can be used to detect the expression of total EGFR by western blot.

Additional Notes

None

Related Categories

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Summary

The Good

Signal is strong and specific.

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

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