ATP Bioluminescent Assay Kit from Sigma

February 12, 2016

BlueskyReddit
Johns Hopkins University
Institute of Genetic Medicine
Postdoctoral Fellow

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Quality of Results

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Company:

Sigma-Aldrich

Product Name:

ATP Assay Kit

Catalog Number:

FLAA-1KT

This kit was used to detect intracellular ATP levels in response to Cetuximab treatment.

Experimental Design and Results Summary

Application

Measurement of intracellular ATP levels

Starting Material

Human cancer cells

Protocol Overview

Cell suspension was mixed with ATP-releasing reagent and ATP levels were measured in 96-well plate.

Tips

None

Results Summary

This kit determines intracellular ATP levels.

Additional Notes

None

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Summary

The Good

Test is quick and easy to perform.

The Bad

Results from luminescent reading has big variance.

The Bottom Line

Generally this kit is good to test intracellular ATP levels and pretty easy to use. However, more replicates are suggested to get reliable results.

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