Phospho-c-Jun (Ser73) Antibody from Cell Signaling

February 10, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-c-Jun (Ser73) Antibody

Catalog Number:

#3270

We used this antibody to detect phosphorylated c-Jun at Ser 73 site in response to 1, 9-Pyrazoloanthrones treatment.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cancer Cells

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

None

Detection

Enhanced chemiluminescence detection kit

Results Summary

We successfully detected phospho-c-Jun at Ser73 site by western blot.

Additional Notes

None

Related Categories

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Summary

The Good

Signal is strong, antibody lasts long after dilution.

The Bad

None

The Bottom Line

This is a perfect antibody for western blot use.

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