Proteintech
YEATS2 Antibody
24717-1-AP
Info: View Product View Product Specs
Browse Similar Products: YEATS2 Western Blot Antibodies
Our lab generates a lot of protein knock outs in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots followed by subsequent sequencing to screen knockout candidates. We chose this antibody because it can be used in many applications and we were interested in performing IP with it later.
Western Blot
Whole cell lysates from mouse ES cells
1:500 dilution overnight in 5% milk buffer at 4C
5% milk in PBST
1:3000 anti-rabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
As you can see from the image, the antibody picked up the same nonspecific bands for all cell lysates, and gave us no useful data. We later confirmed knockout 13 from the image as a true knockout through sequencing.
None
Nothing good about this one
Only nonspecific bands, refund process is complicated and lengthy
Wouldn't recomend