BioLegend APC/Cy7 Anti-Mouse CD45 Review

DBS
University of Montana
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

BioLegend APC/Cy7 anti-mouse CD45

Catalog Number:

103116

We were evaluating cell population frequencies in murine thymuses. Single cell suspensions were made from whole thymuses. Cell populations were assessed through flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary Cells- thymus

Primary Incubation

25 minutes on ice protected from light

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACSAriaII

Results Summary

Overall this antibody gave good separation of peaks without too many compensation issues. The peaks overall looked clean for a tandem dye.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

None

The Bottom Line

It's a good antibody for flow cytometry.

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