Abcam
Anti-Notch1 Antibody [EP1238Y]
ab52627
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Mouse pups were exposed to low oxygen for 5 days, retinal extract were prepared, an equal amount of proteins were resolved in 7.5 % SDS-PAGE, and immunoblotted with anti-Notch1 antibody purchased from Abcam. Selection of antibody was based on specific Notch1 band shown in antibody flyer.
Western Blot
Mouse retinal extract
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degrees Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
Notch-1 antibody has detected only a specific band of Notch-1 protein at nearly 120 kDa molecular weight. However, no or little effect of treatment was observed in Notch-1 levels.
Gives highly specific single band of Notch-1 protein
Excellent antibody