Plasmid Purification

U of Miami
Biology
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

HiSpeed Plasmid Maxi Kit

Catalog Number:

12662

This kit is used to purify large amounts of plasmid. The kit is easy to use and the isolated DNA can be used in downstream applications without further purification.

Experimental Design and Results Summary

Application

Plasmid Purification

Starting Material

None

Protocol Overview

Grow overnight bacteria culture. Spin down and pellet bacteria. Use kit to resuspend cells and then isolate, purify, and elute plasmid DNA.

Tips

Make sure not to overload the plasmid kit

Results Summary

This kit is easy to use and produces large amounts of high quality plasmid DNA with good 260/230 and 260/280 numbers.

Additional Notes

None

Image Gallery

Summary

The Good

High quality plasmid DNA

The Bad

Cost

The Bottom Line

This is a good plasmid purification kit for large plasmid amounts

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