BD Biosciences
PE Mouse Anti-Human CD62L
555544
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The aim of this study was an immunophenotypic analysis of human peripheral blood. This antibody was selected because it promised a good staining using a minimal amount of sample.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated for 30 minutes at room temperature in the dark with the antibody. After this peripheral blood was lysed using the BD lysing buffer as indicated in the manual. Cells were washed three times with PBS and the resuspended in 300ul of FACS buffer and then taken to flow cytometry
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Flow cytometry
This antibody successfully identifies CD62L+ T cells. In the image you see only CD4+ T cells that express CD62L.
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Good staining of CD62L+ T cells
This antibody provides good staining of CD62L+ T cells.