FACS Calibur- Flow Cytometer

Kansas State University
Veterinary Diagnostic Laboratory
RA

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FACS Calibur

Catalog Number:

E4400

The BD FACSCalibur allows users to perform cell analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells, and reticulocytes. It is a 2 laser, 4 color system. It has a 488nm blue laser and 637nm red laser, with 4 filters- 525BP, 585 BP, 670 LP, 650 LP. In our lab, this instrument is primarily used for diagnostic samples.

Experimental Design and Results Summary

Tips

Making sure the cells are not too big or clumpy or it will clog the machine.

Image Gallery

Summary

The Good

Easy to use.

The Bad

Can only do 4 colors.

The Bottom Line

Good instrument for repetitive experiments.

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