Active Caspase 3 in Mouse Skin

February 03, 2016

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

R&D systems

Product Name:

Human/mouse Active Caspase 3 antibody

Catalog Number:

AF835

The aim was to detect active Caspase 3 in mouse skin wounds. This antibody has high background in IF staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin

Primary Incubation

1/1000 dilution, 45min RT

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti-rabbit Alexa fluor 488

Tertiary Incubation

None

Detection

Alexa fluor 488

Results Summary

The tissue is fixed in acetone. The staining has High background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

None

The Bad

High background

The Bottom Line

Not recommended for mouse skin, different fixatives may result in better results.

Share your experience with other scientists. Write a Review! »

Join the discussion