Good General Purpose B Cell Marker

January 22, 2016

Research Participation Program
Oak Ridge Institute for Science and Education
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse/human CD45R/B220

Catalog Number:

103236

We study different methods of activation and inhibition of B cells. We use a variety of anti-B220 clones and colors in our flow cytometry panels. This anti-B220 antibody gives consistent results, strongly labeling mouse splenic B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Live murine splenocytes, red blood cells lysed

Primary Incubation

1:100 dilution of PerCP/Cy5.5 anti-mouse/human CD45R/B220

Blocking Agent

1xPBS+2%FBS+2mM EDTA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR Fortessa

Results Summary

In ungated total mouse splenocytes, the B cells stain positively for surface B220, forming a distinct population from the other splenocytes.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable results, easy to distinguish B220+ from B220- cells.

The Bad

No drawbacks observed.

The Bottom Line

This is a reliable and bright marker for murine splenic B cells. Studying B cells via flow cytometry often requires anti-B220 in different colors to suit the panel, and this is an excellent tool for that purpose.

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