EVOS® FL Auto Imaging System

January 22, 2016

BlueskyReddit
Oregon Health and Science University
Biomedical Engineering
Graduate Research Assistant

Overall

Performance

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

EVOS® FL Auto Imaging System

Catalog Number:

AMAFD1000

The EVOS® FL Auto Imaging System is an easy to use bench top epifluorescence microscope that can be automated to scan multi-well plates, culture flasks and slides. The system features an enormous choice of LED/filter cubes that covers almost all of the current fluorescent dyes and fluorophores. We use the system extensively to scan tumor sections and 96-well plates for high content screening. Overall, the performance is excellent considering the relatively low price point when compared to other microscopes of similar capability.

Experimental Design and Results Summary

Tips

Use the capture nominal focus feature before running a scan to save time on autofocusing and finding sample.

Image Gallery

Summary

The Good

Easy to use LED/filter cubes which require no warm-up time and have significantly longer life with consistent lightning.

The Bad

Image quality is not the best, especially at higher magnification. For high throughput scan, 100X to 200X work the best. There are better high magnification objective lens available but we have not tested them yet.

The Bottom Line

Overall, this system works surprisingly well. We have an Olympus IX-81 Scan^R system for side by side comparison and the performance is generally comparable at 100X and 200X magnifications. Considering the price point, which is about 1/4 of that of the IX-81, this microscope is of good value.

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